热图绘图操作之corplot

2021-06-13 本文已影响0人单细胞空间交响乐

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Corrplot软件包简介

介绍

所述corrplot包是相关矩阵，置信区间的图形显示。它还包含一些进行矩阵重新排序的算法。另外，corrplot擅长细节，包括选择颜色，文本标签，颜色标签，布局等。

可视化方法

有七个可视化方法（参数method中）corrplot包，命名"circle"，"square"，"ellipse"，"number"，"shade"，"color"，"pie"。

正相关以蓝色显示，负相关以红色显示。颜色强度和圆圈的大小与相关系数成正比。

library(corrplot)

## corrplot 0.84 loaded

M <- cor(mtcars)

corrplot(M, method = "circle")

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corrplot(M, method = "square")

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corrplot(M, method = "ellipse")

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corrplot(M, method = "number") # Display the correlation coefficient

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corrplot(M, method = "shade")

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corrplot(M, method = "color")

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corrplot(M, method = "pie")

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布局

共有三种布局类型（参数type）：

"full"（默认）：显示完整的相关矩阵
"upper"：显示相关矩阵的上三角
"lower"：显示相关矩阵的下三角

corrplot(M, type = "upper")

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corrplot.mixed() 是混合可视化样式的包装函数。

corrplot.mixed(M)

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corrplot.mixed(M, lower.col = "black", number.cex = .7)

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corrplot.mixed(M, lower = "ellipse", upper = "circle")

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corrplot.mixed(M, lower = "square", upper = "circle", tl.col = "black")

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重新排序相关矩阵

相关矩阵可以根据相关系数重新排序。这对于确定矩阵中隐藏的结构和图案很重要。有在corrplot（参数四种方法order）的名字命名 "AOE"，"FPC"，"hclust"，"alphabet"。在序列化包中可以找到更多算法。

您还可以通过function手动“重新排序”矩阵corrMatOrder()。

"AOE"一个我ai

a i = { 棕褐色（e i 2 / e i 1），如果 ë 我1 > 0 ;棕褐色（e i 2 / e i 1）+ π ，除此以外。ai={tan⁡(ei2/ei1),if ei1>0;tan⁡(ei2/ei1)+π,otherwise.

Ë 1e1Ë 2e2
"FPC" 对于第一个主成分订单。
"hclust"层次聚类顺序，以及"hclust.method"要使用的聚集方法。"hclust.method"应该是一个 "ward"，"single"，"complete"，"average"，"mcquitty"，"median"或 "centroid"。
"alphabet" 按字母顺序排列。

corrplot(M, order = "AOE")

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corrplot(M, order = "hclust")

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corrplot(M, order = "FPC")

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corrplot(M, order = "alphabet")

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如果使用"hclust"，则corrplot()可以基于层次聚类的结果在相关矩阵图的周围绘制矩形。

corrplot(M, order = "hclust", addrect = 2)

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corrplot(M, order = "hclust", addrect = 3)

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Change background color to lightblue

corrplot(M, type = "upper", order = "hclust",

col = c("black", "white"), bg = "lightblue")

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使用不同的色谱

col1 <- colorRampPalette(c("#7F0000", "red", "#FF7F00", "yellow", "white",
                           "cyan", "#007FFF", "blue", "#00007F"))
col2 <- colorRampPalette(c("#67001F", "#B2182B", "#D6604D", "#F4A582",
                           "#FDDBC7", "#FFFFFF", "#D1E5F0", "#92C5DE",
                           "#4393C3", "#2166AC", "#053061"))
col3 <- colorRampPalette(c("red", "white", "blue")) 
col4 <- colorRampPalette(c("#7F0000", "red", "#FF7F00", "yellow", "#7FFF7F",
                           "cyan", "#007FFF", "blue", "#00007F"))
whiteblack <- c("white", "black")
## using these color spectra
corrplot(M, order = "hclust", addrect = 2, col = col1(100))

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corrplot(M, order = "hclust", addrect = 2, col = col2(50))

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corrplot(M, order = "hclust", addrect = 2, col = col3(20))

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corrplot(M, order = "hclust", addrect = 2, col = col4(10))

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corrplot(M, order = "hclust", addrect = 2, col = whiteblack, bg = "gold2")

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还可以使用标准调色板（包grDevices）

corrplot(M, order = "hclust", addrect = 2, col = heat.colors(100))

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corrplot(M, order = "hclust", addrect = 2, col = terrain.colors(100))

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corrplot(M, order = "hclust", addrect = 2, col = cm.colors(100))

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corrplot(M, order = "hclust", addrect = 2, col = gray.colors(100))

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其他选择是使用RcolorBrewer包。

library(RColorBrewer)

corrplot(M, type = "upper", order = "hclust",

col = brewer.pal(n = 8, name = "RdBu"))

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corrplot(M, type = "upper", order = "hclust",

col = brewer.pal(n = 8, name = "RdYlBu"))

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corrplot(M, type = "upper", order = "hclust",

col = brewer.pal(n = 8, name = "PuOr"))

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更改文本标签和图例的颜色和旋转

参数cl.*用于颜色图例，tl.*如果用于文本图例。对于文本标签，tl.col（文本标签颜色）和tl.srt（文本标签字符串旋转）用于更改文本颜色和旋转。

这里有些例子。

## remove color legend and text legend 

corrplot(M, order = "AOE", cl.pos = "n", tl.pos = "n")

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## bottom  color legend, diagonal text legend, rotate text label

corrplot(M, order = "AOE", cl.pos = "b", tl.pos = "d", tl.srt = 60)

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## a wider color legend with numbers right aligned

corrplot(M, order = "AOE", cl.ratio = 0.2, cl.align = "r")

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 ## text labels rotated 45 degrees

corrplot(M, type = "lower", order = "hclust", tl.col = "black", tl.srt = 45)

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处理非相关矩阵

corrplot(abs(M),order = "AOE", col = col3(200), cl.lim = c(0, 1))

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## visualize a  matrix in [-100, 100]

ran <- round(matrix(runif(225, -100,100), 15))

corrplot(ran, is.corr = FALSE, method = "square")

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## a beautiful color legend 

corrplot(ran, is.corr = FALSE, method = "ellipse", cl.lim = c(-100, 100))

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如果矩阵是矩形，则可以使用win.asp参数调整纵横比，以使矩阵呈现为正方形。

ran <- matrix(rnorm(70), ncol = 7)

corrplot(ran, is.corr = FALSE, win.asp = .7, method = "circle")

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处理缺失（NA）值

默认情况下，corrplot将NA值呈现为"?"字符。使用na.label 参数，可以使用不同的值（最多支持两个字符）。

M2 <- M

diag(M2) = NA

corrplot(M2)

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corrplot(M2, na.label = "o")

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corrplot(M2, na.label = "NA")

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在标签中使用“ plotmath”表达式

从version开始0.78，可以在变量名称中使用 plotmath表达式。要激活plotmath渲染，前缀的人物之一的标签":"，"="或"$"。

M2 <- M[1:5,1:5]

colnames(M2) <- c("alpha", "beta", ":alpha+beta", ":a[0]", "=a[beta]")

rownames(M2) <- c("alpha", "beta", NA, "$a[0]", "$ a[beta]")

corrplot(M2)

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将相关图与显着性检验相结合

res1 <- cor.mtest(mtcars, conf.level = .95)

res2 <- cor.mtest(mtcars, conf.level = .99)

 ## specialized the insignificant value according to the significant level

corrplot(M, p.mat = res1$p, sig.level = .2)

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corrplot(M, p.mat = res1$p, sig.level = .05)

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corrplot(M, p.mat = res1$p, sig.level = .01)

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## leave blank on no significant coefficient

corrplot(M, p.mat = res1$p, insig = "blank")

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 ## add p-values on no significant coefficient

corrplot(M, p.mat = res1$p, insig = "p-value")

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## add all p-values

corrplot(M, p.mat = res1$p, insig = "p-value", sig.level = -1)

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 ## add cross on no significant coefficient 

corrplot(M, p.mat = res1$p, order = "hclust", insig = "pch", addrect = 3)

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可视化置信区间

corrplot(M, low = res1$lowCI, upp = res1$uppCI, order = "hclust",

rect.col = "navy", plotC = "rect", cl.pos = "n")

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corrplot(M, p.mat = res1$p, low = res1$lowCI, upp = res1$uppCI,

order = "hclust", pch.col = "red", sig.level = 0.01,

addrect = 3, rect.col = "navy", plotC = "rect", cl.pos = "n")

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res1 <- cor.mtest(mtcars, conf.level = .95)

corrplot(M, p.mat = res1$p, insig = "label_sig",

sig.level = c(.001, .01, .05), pch.cex = .9, pch.col = "white")

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corrplot(M, p.mat = res1$p, method = "color",

insig = "label_sig", pch.col = "white")

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corrplot(M, p.mat = res1$p, method = "color", type = "upper",

sig.level = c(.001, .01, .05), pch.cex = .9,

insig = "label_sig", pch.col = "white", order = "AOE")

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corrplot(M, p.mat = res1$p, insig = "label_sig", pch.col = "white",

pch = "p<.05", pch.cex = .5, order = "AOE")

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自定义相关图

# matrix of the p-value of the correlation

p.mat <- cor.mtest(mtcars)$p

head(p.mat[, 1:5])

##              [,1]         [,2]         [,3]         [,4]         [,5]

## [1,] 0.000000e+00 6.112687e-10 9.380327e-10 1.787835e-07 1.776240e-05

## [2,] 6.112687e-10 0.000000e+00 1.802838e-12 3.477861e-09 8.244636e-06

## [3,] 9.380327e-10 1.802838e-12 0.000000e+00 7.142679e-08 5.282022e-06

## [4,] 1.787835e-07 3.477861e-09 7.142679e-08 0.000000e+00 9.988772e-03

## [5,] 1.776240e-05 8.244636e-06 5.282022e-06 9.988772e-03 0.000000e+00

## [6,] 1.293959e-10 1.217567e-07 1.222320e-11 4.145827e-05 4.784260e-06

# Specialized the insignificant value according to the significant level

corrplot(M, type = "upper", order = "hclust", 

p.mat = p.mat, sig.level = 0.01)

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# Leave blank on no significant coefficient

corrplot(M, type = "upper", order = "hclust", 

p.mat = p.mat, sig.level = 0.01, insig = "blank")

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在上图中，p值> 0.01的相关被认为是无关紧要的。在这种情况下，相关系数值留为空白或添加叉号。

col <- colorRampPalette(c("#BB4444", "#EE9988", "#FFFFFF", "#77AADD", "#4477AA"))

corrplot(M, method = "color", col = col(200),

type = "upper", order = "hclust", number.cex = .7,

addCoef.col = "black", # Add coefficient of correlation

tl.col = "black", tl.srt = 90, # Text label color and rotation

# Combine with significance

p.mat = p.mat, sig.level = 0.01, insig = "blank", 

# hide correlation coefficient on the principal diagonal

diag = FALSE)

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探索大型功能矩阵

# generating large feature matrix (cols=features, rows=samples)

num_features <- 60 # how many features

num_samples <- 300 # how many samples

DATASET <- matrix(runif(num_features * num_samples),

 nrow = num_samples, ncol = num_features)

# setting some dummy names for the features e.g. f23

colnames(DATASET) <- paste0("f", 1:ncol(DATASET))

 # let's make 30% of all features to be correlated with feature "f1"

 num_feat_corr <- num_features * .3

 idx_correlated_features <- as.integer(seq(from = 1,

to = num_features,

 length.out = num_feat_corr))[-1]

 for (i in idx_correlated_features) {

DATASET[,i] <- DATASET[,1] + runif(num_samples) # adding some noise

  }

 corrplot(cor(DATASET), diag = FALSE, order = "FPC",

tl.pos = "td", tl.cex = 0.5, method = "color", type = "upper")

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生活很好，等你超越

热图绘图操作之corplot

放假期间我们学习一下基础的东西

Corrplot软件包简介

介绍

可视化方法

布局

重新排序相关矩阵

Change background color to lightblue

使用不同的色谱

更改文本标签和图例的颜色和旋转

处理非相关矩阵

处理缺失（NA）值

在标签中使用“ plotmath”表达式

将相关图与显着性检验相结合

可视化置信区间

自定义相关图

探索大型功能矩阵

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