Discovery of microarray-identified genes associated with ovarian cancer progression

提出问题：

mRNA expression profiles与ovarian cancer progression有什么联系？

检索数据库：

Oncomine、GEO、 cBioPortal for Cancer Genomics

检索内容：

1、Microarrays GDS3592, GDS3297, GDS2785 and GDS3894 from Oncomine

2、The clinical data of 489 cases of ovarian carcinoma with gene expression value in TCGA

参数设置：

P-values are corrected for multiple comparisons

输出结果：

1、Enrichment of the biological processes of a group of genes

2、Annotation of biological processes with genes

3、 protein/gene-protein/gene interaction analysis

4、Homogeneity of the variances

5、probability of survival and significance

分析软件：

1、DAVID online tool

2、Coremine Medical online database

3、 GeneMANIA online tool

4、 SPSS 20.0 software

结果验证：

1、细胞：The human epithelial ovarian cancer A2780 and SKOV3 cells、Cisplatin-resistant cell line A2780-DDP and SKOV3-DDP, and carboplatin-resistant cell line A2780-CBP。

2、RT-PCR