此教程无需R语言

Step 1 确定需要检索的芯片

例如 GSE33006

Step 2 利用GEO2R分析差异基因

GEO2R https://www.ncbi.nlm.nih.gov/geo/geo2r/

Quick start

• Specify a GEO Series accession and a Platform if prompted.
• Click 'Define groups' and enter names for the groups of Samples you plan to compare, e.g., test and control.
• Assign Samples to each group. Highlight Sample rows then click the group name to assign those Samples to the group. Use the Sample metadata (title, source and characteristics) columns to help determine which Samples belong to which group.
• Click 'Top 250' to perform the calculation with default settings.
• Results are presented as a table of genes ordered by significance. The top 250 genes are presented and may be viewed as profile graphs. Alternatively, the complete results table may be saved.
• You may change settings in Options tab.
  右键存为txt格式

Step 3 差异基因数据导入excel

获取数据（从文件获取）

Step 4 利用网站制作维恩图

http://bioinformatics.psb.ugent.be/webtools/Venn/

图片.png
从Excel筛选P值小于阈值的基因名称（Gene.symbo）
录入后点subimit
得到维恩图