Linux: 根据 ID 提取 fasta

2023-09-18  本文已影响0人  LET149

perl脚本根据ID提取fasta序列文件

perl id2fasta input.fasta ID.txt output.fasta

#!/bin/perl
#unless(@ARGV==3){
#   die "usage: $0 <input.fa> <lst> <output.fas>\n";
#}
$file=shift;
$lst=shift;
$out=shift;

open FILE,$file;
open LST,$lst;
open OUT,">".$out;

while(<FILE>){
    chomp;
    my $line=$_;
    if($line=~/^>/){
        my @line=split /[ |\t]/,$line;
        our $name=$line[0];
        #print "$name\n";
    }
    else{
        $seq_hash{$name}.=$line;
    }
}
while(<LST>){
    chomp;
    my $line=$_;
    if($line=~/^>/){
        $ID=$line;
        if(exists $seq_hash{$ID}){
            print OUT "$ID\n$seq_hash{$ID}\n";
        }
        else{
            print OUT "error1: ".$ID." no found.\n";
        }
    }
    else{
        my $ID=">".$line;
        if(exists $seq_hash{$ID}){
            print OUT "$ID\n$seq_hash{$ID}\n";
        }
        else{
            print OUT "error2: ".$ID." no found.\n";
        }
    }
}
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