好好学!这技术值一篇Plant physiology!
2021-05-14 本文已影响0人
邵扬_Barnett
今天看到Plant physiology在线发表了一篇文章。
In this study, we developed a software package, named GridFree, to overcome these challenges. GridFree uses an unsupervised machine learning approach, K-Means, to segment kernels from the background by using principal component analysis on both raw image channels and their color indices.
看完我满脑子的就这?难道不是用image J就能搞定了么?
点这里进入官网,我惊了。GRIDFREE (zzlab.net)
就这??
我不禁陷入沉思,是Image J不骚了还是OpenCV提不动刀了?这重复造轮子还能发到一区的。
好好看,好好学。包你5分钟学会价值一篇PP的技术。
首先,去下载FIJI。什么是FIJI? FIJI就是Image J啊!(Fiji is Image J)
然后选择split channel,复制一层红色通道
然后通道阈值选取种子,记得勾选黑色背景
之后选择Process->Binary->watershed
然后Process->Find Maxima
别忘了选择浅色背景
一篇PP文章的技术你们学废了嘛?
最后右上角有一个被分成了两个怎么办?你用画笔涂黑就好了呀……