好好学!这技术值一篇Plant physiology!
2021-05-14 本文已影响0人
邵扬_Barnett
今天看到Plant physiology在线发表了一篇文章。
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In this study, we developed a software package, named GridFree, to overcome these challenges. GridFree uses an unsupervised machine learning approach, K-Means, to segment kernels from the background by using principal component analysis on both raw image channels and their color indices.
看完我满脑子的就这?难道不是用image J就能搞定了么?
点这里进入官网,我惊了。GRIDFREE (zzlab.net)
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就这??
我不禁陷入沉思,是Image J不骚了还是OpenCV提不动刀了?这重复造轮子还能发到一区的。
好好看,好好学。包你5分钟学会价值一篇PP的技术。
首先,去下载FIJI。什么是FIJI? FIJI就是Image J啊!(Fiji is Image J)
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然后选择split channel,复制一层红色通道
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然后通道阈值选取种子,记得勾选黑色背景
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之后选择Process->Binary->watershed
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然后Process->Find Maxima
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别忘了选择浅色背景
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一篇PP文章的技术你们学废了嘛?
最后右上角有一个被分成了两个怎么办?你用画笔涂黑就好了呀……
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