RunPCA时遇到error in irlba(A = t(x

> pbmc <- RunPCA(pbmc, features = VariableFeatures(object = pbmc))

Error in irlba(A = t(x = object), nv = npcs, ...) : max(nu, nv) must be strictly less than min(nrow(A), ncol(A))        

简单来说就是你的细胞数小于50。
npcs的选择是默认为50。PC当然要小于矩阵的行数或列数中的最小值。所以只需修改默认值即可。

解决方案：

pbmc <- RunPCA(pbmc, npcs = 33(填“细胞数-1”）,features = VariableFeatures(object = pbmc))