blast输出格式可以用outfmt控制

-outfmt <String>

alignment view options:

0 = Pairwise,

1 = Query-anchored showing identities,

2 = Query-anchored no identities,

3 = Flat query-anchored showing identities,

4 = Flat query-anchored no identities,

5 = BLAST XML,

6 = Tabular,

7 = Tabular with comment lines,

8 = Seqalign (Text ASN.1),

9 = Seqalign (Binary ASN.1),

10 = Comma-separated values,

11 = BLAST archive (ASN.1),

12 = Seqalign (JSON),

13 = Multiple-file BLAST JSON,

14 = Multiple-file BLAST XML2,

15 = Single-file BLAST JSON,

16 = Single-file BLAST XML2,

17 = Sequence Alignment/Map (SAM),

18 = Organism Report

Options 6, 7, 10 and 17 can be additionally configured to produce a custom format specified by space delimited format specifiers, or in the case of options 6, 7, and 10, by a token specified by the delim keyword. E.g.: "17 delim=@ qacc sacc score". The delim keyword must appear after the numeric output format specification.

其中常用的是6、7两种格式

另外6、7、10、17格式可以调整输出内容

常用的是qseqid、sseqid：序列名称和数据库中序列名称

length、qlen、slen、nident、mismatch、positive、gaps：表示不同的比对的长度

sstart、send、qstart、qend：起始结束位点

sseq、qseq、qframe、sframe、frames：输出序列

pident、nident、evalue、bitscore、qcovs、qcovhsp、qcovus：判断序列比对质量

通过这些格式输出可以帮助高效进行序列比对和注释，之后再补充除6、7、10

17外其他几种序列输出格式。