getorganelle组装报错

2024-02-17  本文已影响0人  多啦A梦的时光机_648d

get_org.log.txt日志文件

2024-02-18 14:17:41,553 - INFO: Assembling finished.

2024-02-18 14:17:46,341 - ERROR: Slimming 218/extended_spades/K105/assembly_graph.fastg failed. Please check 218/extended_spades/K105/slim.log.txt for details. 
2024-02-18 14:17:54,173 - ERROR: Slimming 218/extended_spades/K85/assembly_graph.fastg failed. Please check 218/extended_spades/K85/slim.log.txt for details. 
2024-02-18 14:18:35,218 - ERROR: Slimming 218/extended_spades/K65/assembly_graph.fastg failed. Please check 218/extended_spades/K65/slim.log.txt for details. 
2024-02-18 14:19:19,922 - ERROR: Slimming 218/extended_spades/K45/assembly_graph.fastg failed. Please check 218/extended_spades/K45/slim.log.txt for details. 
2024-02-18 14:19:19,922 - ERROR: No valid assembly graph found!
2024-02-18 14:19:19,923 - WARNING: This might due to a damaged dependency, to unreasonable seed/parameter choices, or to a bug.
2024-02-18 14:19:19,923 - INFO: Please first search similar issues at https://github.com/Kinggerm/GetOrganelle/issues, then leave your message following the same issue, or open an issue at https://github.com/Kinggerm/GetOrganelle/issues if it is new, Please always attach the get_org.log.txt file.

导致无法成环,查看extended_spades/K105下面的slim.log.txt日志,

2024-02-18 14:17:43,164 - INFO: Slimming file 1/1: 218/extended_spades/K105/assembly_graph.fastg
2024-02-18 14:17:45,990 - INFO: Parsing input finished.
2024-02-18 14:17:46,131 - INFO: Preparing fasta file finished.
2024-02-18 14:17:46,131 - INFO: Executing BLAST to /home/lx_sky6/.GetOrganelle/LabelDatabase/embplant_pt ...
2024-02-18 14:17:46,131 - INFO: Executing BLAST ...
2024-02-18 14:17:46,154 - ERROR: BLAST Database error: Error: Not a valid version 4 database.

发现BLAST报错,解决办法,升级blast,我查看了一下自己的blast是2.5版本,升级到最新2.15即可解决。

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