官网：
https://support.10xgenomics.com/single-cell-gene-expression/

系统要求：
Linux 至少8核intel或AMD CPU(推荐16核)
64GB RAM(推荐128GB)，最低配的64G允许cellranger aggr合并最多250k个细胞
1T硬盘
64-bit CentOS/RedHat 6.0 or Ubuntu 12.04

1.下载

1.1 下载cellranger

https://support.10xgenomics.com/single-cell-gene-expression/software/downloads/latest
填写信息后，会给出两个软件（v3.1）下载链接，以及参考基因组（v3.0）

$ curl -o cellranger-3.1.0.tar.gz "http://cf.10xgenomics.com/releases/cell-exp/cellranger-3.1.0.tar.gz?Expires=1572780358&Policy=eyJTdGF0ZW1lbnQiOlt7IlJlc291cmNlIjoiaHR0cDovL2NmLjEweGdlbm9taWNzLmNvbS9yZWxlYXNlcy9jZWxsLWV4cC9jZWxscmFuZ2VyLTMuMS4wLnRhci5neiIsIkNvbmRpdGlvbiI6eyJEYXRlTGVzc1RoYW4iOnsiQVdTOkVwb2NoVGltZSI6MTU3Mjc4MDM1OH19fV19&Signature=DtuIA6Dyzv22DB7LfJAqlWhytmcAEHgEVzSR9YgQnkbW91MkFvorTSoOW2wFjRtF9NytOUdeA4M~oFci9su1Yh-3ZTKR27~qaZ-neywY96OppGtqcsgyr6iiWi91bICHOvkD5pVUrVsf0-u5rz9kd0NxltWCoEeVz8EECsocEnlYDL5SG1F1ykFn~WxQPvgX67puz816A0cN69jRDHMdzR4d3mVxj72SmXT193Y5r0UF0Kamyqovb1JRLAl~bz6Km8fomjBOSXlwGQIFllzAyjRHbQPCmisE3YnoetCW1sRl1-yDtYoRp1N16fP~qP8t8pWP-XEtBlhMFNR-~ZGSeg__&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA"

wget -O cellranger-3.1.0.tar.gz "http://cf.10xgenomics.com/releases/cell-exp/cellranger-3.1.0.tar.gz?Expires=1592100490&Policy=eyJTdGF0ZW1lbnQiOlt7IlJlc291cmNlIjoiaHR0cDovL2NmLjEweGdlbm9taWNzLmNvbS9yZWxlYXNlcy9jZWxsLWV4cC9jZWxscmFuZ2VyLTMuMS4wLnRhci5neiIsIkNvbmRpdGlvbiI6eyJEYXRlTGVzc1RoYW4iOnsiQVdTOkVwb2NoVGltZSI6MTU5MjEwMDQ5MH19fV19&Signature=BpMfSadAt6tlGTtvpZ64yan4xioUDQl1dZfuQA11kQEPE1xzBPT-XeZu2Ap-ptF7KebFseVxCEn2xN6vzPcwST0gnPOVGlgPBdgseslLaVO23dnAAzoNwSp86TX0Jo6w4XqzdUjLQ~DcCEJWBIomSYvJ6Q9DFXx9OTO751Q-tnkqZ~dLs~j6J16NGv4A4bjzMOe00yxC7GixDLii~Ge6rPsQHdAtFcgB2lhx8OKLzKR5v9N50IQdDtYhT1IuWwnk~Z7mTIBWPFNl~KSwg7Hen6LGhb0IBCR0Ekpa17BOYq0e8cLOykRXo5orOPdrZwff7uDc~rpipNwJZjJ31an5Jw__&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA"

如果要下载之前的版本，点击右下角红色的“Downloads v3.1(latest)”,选择对应的版本。

1.2 下载基因组

GRCh38

wget http://cf.10xgenomics.com/supp/cell-exp/refdata-cellranger-GRCh38-3.0.0.tar.gz

或

curl -O http://cf.10xgenomics.com/supp/cell-exp/refdata-cellranger-GRCh38-3.0.0.tar.gz

mm10

curl -O http://cf.10xgenomics.com/supp/cell-exp/refdata-cellranger-mm10-3.0.0.tar.gz

或

wget http://cf.10xgenomics.com/supp/cell-exp/refdata-cellranger-mm10-3.0.0.tar.gz

也可以通过点击Download进行网页下载，或者点击“Build steps”查看每一个文件详细的构建过程。其中包括cellranger软件的两个命令：
cellranger mkgtf 和 cellranger mkref

2. 解压

 tar zxvf cellranger-3.1.0.tar.gz

3. 添加环境变量

echo 'export PATH=./luozhixin/cellranger/cellranger-3.1.0:$PATH' >> ~/.bashrc

3.1测试

cellranger