【包】seurat-2 多组学联合

2022-10-30  本文已影响0人  JamesMori

写在前面:看官方文档,很可能会误认为很简单,或者有些人直接延用官方的路径。这都是不可取的,最重要的永远是自己的思路,不仅是科研方面的,还是代码绘图方面的。官方的绘图函数虽然比较漂亮,但是针对性很强,学习底层的绘图对个人意义才是最大的。官方的东西借鉴和学习。
有哪些组学呢?
有哪些单细胞多组学联用技术呢?

CITE-seq:Simultaneous epitope and transcriptome measurement in single cells | Nature Methods
10x multiome kit:Single Cell Multiome ATAC + Gene Expression - 10x Genomics
Cell Hashing:Cell Hashing with barcoded antibodies enables multiplexing and doublet detection for single cell genomics | Genome Biology | Full Text (biomedcentral.com)

总之就是转录组+表面蛋白、ATAC、甲基化组、基因组。读文章再补充
多组学表面上是多层次,根本上可以解释为时间顺序上的故事,是可以互为因果的。
seurat这里有个Weighted Nearest Neighbors (WNN) 方法,根据多组学结合对细胞作聚类,有个加权过程。
Weighted Nearest Neighbor Analysis • Seurat (satijalab.org)
!但是这个文章并没有用WNN

1. 样本数据准备

1.1. 提取数据:8,617 cord blood mononuclear cells (CBMCs)

RNA和11个antibody-derived tags (ADT)表面蛋白

# Load in the RNA UMI matrix

# Note that this dataset also contains ~5% of mouse cells, which we can use as negative
# controls for the protein measurements. For this reason, the gene expression matrix has
# HUMAN_ or MOUSE_ appended to the beginning of each gene.
cbmc.rna <- as.sparse(read.csv(file = "../data/GSE100866_CBMC_8K_13AB_10X-RNA_umi.csv.gz", sep = ",",header = TRUE, row.names = 1))

# To make life a bit easier going forward, we're going to discard all but the top 100 most
# highly expressed mouse genes, and remove the 'HUMAN_' from the CITE-seq prefix
cbmc.rna <- CollapseSpeciesExpressionMatrix(cbmc.rna)

# Load in the ADT UMI matrix
cbmc.adt <- as.sparse(read.csv(file = "../data/GSE100866_CBMC_8K_13AB_10X-ADT_umi.csv.gz", sep = ",", header = TRUE, row.names = 1))

# Note that since measurements were made in the same cells, the two matrices have identical column names
#检验永远是最重要哒
all.equal(colnames(cbmc.rna), colnames(cbmc.adt))

CollapseSpeciesExpressionMatrix(
  object,
  prefix = "HUMAN_",
  controls = "MOUSE_",
  ncontrols = 100
)
#这些小工具真的太有意思了

1.2. 创建seurat 对象

# creates a Seurat object based on the scRNA-seq data
cbmc <- CreateSeuratObject(counts = cbmc.rna)

# We can see that by default, the cbmc object contains an assay storing RNA measurement
Assays(cbmc) #seurat有很多query,可以queryseurat对象的某类数据,相当于seurat的子对象
## [1] "RNA"

Query Specific Object Types — Assays • SeuratObject (mojaveazure.github.io)

#assay子对象
# create a new assay to store ADT information
adt_assay <- CreateAssayObject(counts = cbmc.adt)

# add this assay to the previously created Seurat object
cbmc[["ADT"]] <- adt_assay

# Validate that the object now contains multiple assays
Assays(cbmc)
## [1] "RNA" "ADT"

# Extract a list of features measured in the ADT assay
rownames(cbmc[["ADT"]])
##  [1] "CD3"    "CD4"    "CD8"    "CD45RA" "CD56"   "CD16"   "CD10"   "CD11c" 
##  [9] "CD14"   "CD19"   "CD34"   "CCR5"   "CCR7"


# Note that we can easily switch back and forth between the two assays to specify the default
# for visualization and analysis

# List the current default assay
DefaultAssay(cbmc)
## [1] "RNA"
# Switch the default to ADT
DefaultAssay(cbmc) <- "ADT"
DefaultAssay(cbmc)
## [1] "ADT"

2. 根据scRNA-seq进行细胞聚类

# Note that all operations below are performed on the RNA assay Set and verify that the
# default assay is RNA
DefaultAssay(cbmc) <- "RNA"

紧接着就是一连串的标准流程,可以【包】seurat-1 回顾,下面代码有简单的注释

# perform visualization and clustering steps
cbmc <- NormalizeData(cbmc)#标准化数据
cbmc <- FindVariableFeatures(cbmc)#筛选高变异性基因
cbmc <- ScaleData(cbmc)#归一化高变异性基因
cbmc <- RunPCA(cbmc, verbose = FALSE)#线性分解细胞对高变异基因的差异解释度
cbmc <- FindNeighbors(cbmc, dims = 1:30)#细胞间距离
cbmc <- FindClusters(cbmc, resolution = 0.8, verbose = FALSE)#细胞聚类
cbmc <- RunUMAP(cbmc, dims = 1:30)#非线性展示低维细胞聚类
DimPlot(cbmc, label = TRUE)#绘图

标准化与归一化:标准化是去除样本间基线差异、归一化是去除参数间权重差异

聚类结果

3. 多组学切换,灵活可视化

# Normalize ADT data,
DefaultAssay(cbmc) <- "ADT"
cbmc <- NormalizeData(cbmc, normalization.method = "CLR", margin = 2)
DefaultAssay(cbmc) <- "RNA"

# Note that the following command is an alternative but returns the same result
cbmc <- NormalizeData(cbmc, normalization.method = "CLR", margin = 2, assay = "ADT")

# Now, we will visualize CD14 levels for RNA and protein By setting the default assay, we can
# visualize one or the other
DefaultAssay(cbmc) <- "ADT"
p1 <- FeaturePlot(cbmc, "CD19", cols = c("lightgrey", "darkgreen")) + ggtitle("CD19 protein")
DefaultAssay(cbmc) <- "RNA"
p2 <- FeaturePlot(cbmc, "CD19") + ggtitle("CD19 RNA")

# place plots side-by-side
p1 | p2
# Alternately, we can use specific assay keys to specify a specific modality Identify the key
# for the RNA and protein assays
Key(cbmc[["RNA"]])
## [1] "rna_"
Key(cbmc[["ADT"]])
## [1] "adt_"
# Now, we can include the key in the feature name, which overrides the default assay
p1 <- FeaturePlot(cbmc, "adt_CD19", cols = c("lightgrey", "darkgreen")) + ggtitle("CD19 protein")
p2 <- FeaturePlot(cbmc, "rna_CD19") + ggtitle("CD19 RNA")
p1 | p2
feature图

4. markers鉴定

# as we know that CD19 is a B cell marker, we can identify cluster 6 as expressing CD19 on the
# surface
VlnPlot(cbmc, "adt_CD19")
小提琴图
# we can also identify alternative protein and RNA markers for this cluster through
# differential expression
adt_markers <- FindMarkers(cbmc, ident.1 = 6, assay = "ADT")
rna_markers <- FindMarkers(cbmc, ident.1 = 6, assay = "RNA")

head(adt_markers)
##                p_val avg_log2FC pct.1 pct.2     p_val_adj
## CD19   2.067533e-215  1.2787751     1     1 2.687793e-214
## CD45RA 8.106076e-109  0.4117172     1     1 1.053790e-107
## CD4    1.123162e-107 -0.7255977     1     1 1.460110e-106
## CD14   7.212876e-106 -0.5060496     1     1 9.376739e-105
## CD3     1.639633e-87 -0.6565471     1     1  2.131523e-86
## CD8     1.042859e-17 -0.3001131     1     1  1.355716e-16

head(rna_markers)
##       p_val avg_log2FC pct.1 pct.2 p_val_adj
## BANK1     0   1.963277 0.456 0.015         0
## CD19      0   1.563124 0.351 0.004         0
## CD22      0   1.503809 0.284 0.007         0
## CD79A     0   4.177162 0.965 0.045         0
## CD79B     0   3.774579 0.944 0.089         0
## FCRL1     0   1.188813 0.222 0.002         0

5. 其他绘图

# Draw ADT scatter plots (like biaxial plots for FACS). Note that you can even 'gate' cells if
# desired by using HoverLocator and FeatureLocator
FeatureScatter(cbmc, feature1 = "adt_CD19", feature2 = "adt_CD3")
feature dot1
# view relationship between protein and RNA
FeatureScatter(cbmc, feature1 = "adt_CD3", feature2 = "rna_CD3E")
feature dot2
#对seurat数据格式要熟悉一点
# Let's look at the raw (non-normalized) ADT counts. You can see the values are quite high,
# particularly in comparison to RNA values. This is due to the significantly higher protein
# copy number in cells, which significantly reduces 'drop-out' in ADT data
FeatureScatter(cbmc, feature1 = "adt_CD4", feature2 = "adt_CD8", slot = "counts")
feature dot3

6. 小结

这个文章只是最基础的多组学联合分析,甚至都没涉及相关性计算。文章末尾也写了更多阅读的链接:

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