Cell Ranger安装

2022-09-14  本文已影响0人  oceanandshore

Cell Ranger安装很简单,搜一下教程有很多,但是要注意的是记得添加环境变量 这一步很多教程没提,注意一下。
步骤就是 下载——解压——添加环境变量 就安装好了

# 2.0版本下载
curl -o cellranger-2.0.2.tar.gz "http://cf.10xgenomics.com/releases/cell-exp/cellranger-2.0.2.tar.gz?Expires=1557256518&Policy=eyJTdGF0ZW1lbnQiOlt7IlJlc291cmNlIjoiaHR0cDovL2NmLjEweGdlbm9taWNzLmNvbS9yZWxlYXNlcy9jZWxsLWV4cC9jZWxscmFuZ2VyLTIuMC4yLnRhci5neiIsIkNvbmRpdGlvbiI6eyJEYXRlTGVzc1RoYW4iOnsiQVdTOkVwb2NoVGltZSI6MTU1NzI1NjUxOH19fV19&Signature=HoJUuPo4iTFdQgzFU1GH7uKf3uGitQxTjB6WOA9qGPlejf7tNcBPjO65WuSUZ~w8WWdeAvky-oV7XGfheY-bUr2b7QHr7jQEqc84cyU~PLvT~fYjkgC7cG7nlpbJOT~b7U~YH9amvR~SCLlyynp7scPDIA~9~keCYrIPgevTf2QyktybuSyjNTwugefOic~~XFkc9lrS~WQ9MNA1CLl4ExlQKsxWS77PEB6mwrMZXX65obDnZW9fIs3dIny6H5YoadbkgmsT52jmLien6PsG1g2jpAO90pPuHoru8LL64Q9gmB3I0nJAqi3EmrO3GKnUpHUhGb6doKmjSN6XccpmsA__&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA"
# 2.1版本下载
curl -o cellranger-2.1.1.tar.gz "http://cf.10xgenomics.com/releases/cell-exp/cellranger-2.1.1.tar.gz?Expires=1557260110&Policy=eyJTdGF0ZW1lbnQiOlt7IlJlc291cmNlIjoiaHR0cDovL2NmLjEweGdlbm9taWNzLmNvbS9yZWxlYXNlcy9jZWxsLWV4cC9jZWxscmFuZ2VyLTIuMS4xLnRhci5neiIsIkNvbmRpdGlvbiI6eyJEYXRlTGVzc1RoYW4iOnsiQVdTOkVwb2NoVGltZSI6MTU1NzI2MDExMH19fV19&Signature=RNQd-gTASTQhtnUSBfQWrnqo6Pyy2wDXtV5tlxkG97727GvoRhMqFXbEsz4gJl2BMckdVvW3S1tZRwRo5pmxPzmhq-8RKxf99pGqlzo84HYqhbIRkxXlIbLbj-u3PUJqo8cesWpbSVSKkS2TCNS-9GMFNieQswqMS2-DN4BqoBOJnWr7T4wlOMd9hypXWwOsW2P2fqaM-WP2ooMyo-oIxm3y9gDghXdDEP5lvHU7GCQcFGGexkdIrD6S5p8JPJ1DB5XieGrtEuP1YVp6tLMGXFoRWXS8dQLI1egWDYlOuRaiQgLIb3o5ZxBg5NpzLPP5kDHMAVzJFdBpf~~rkyNYTA__&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA"
# 3.1.0版本下载
curl -o cellranger-3.1.0.tar.gz "http://cf.10xgenomics.com/releases/cell-exp/cellranger-3.1.0.tar.gz?Expires=1592533436&Policy=eyJTdGF0ZW1lbnQiOlt7IlJlc291cmNlIjoiaHR0cDovL2NmLjEweGdlbm9taWNzLmNvbS9yZWxlYXNlcy9jZWxsLWV4cC9jZWxscmFuZ2VyLTMuMS4wLnRhci5neiIsIkNvbmRpdGlvbiI6eyJEYXRlTGVzc1RoYW4iOnsiQVdTOkVwb2NoVGltZSI6MTU5MjUzMzQzNn19fV19&Signature=devRIwYTTAupcAWpv2pvGblUKXeqwRmRO~cp49UGz5XTM~4vzzGGS63ZwpZ8VnDy5wjbv27ydnx19jN~xyIE9dkr8XFnhTNNgs3G9AYC72oiCTbv1j38ohEy9hfoVoFCl7x5b1-4DG-~SaIggBIDJB0ADLn28xGuyoIKPpuc69hdTEzbriFDdK-596SIKGT-ljXyCddreC81RDuPQs5wARicScA058E7A4HD~5R9iPew1ZxCjkPmD7PQtg976zJA3AOtJDPDeLQM-Xps~DgbpuHvRqEYA~GTP8lTX5icO4VOcd24btgHOb5Qo2jQDb5VtHJeMlSH6mTzz4jFhZ8vBQ__&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA"
# 解压 
tar -zxvf cellranger-2.0.2.tar.gz 
# 添加环境变量
echo 'export PATH=/home/biosoft/cellranger-2.2.0:$PATH' >> ~/.bashrc
source  ~/.bashrc

参考:
CellRanger使用学习
Cell Ranger的安装与配置

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