生信星球培训第139期

学习小组day3笔记--清南北南

2022-05-10  本文已影响0人  清南北南

今天也是记流水账的一天,下载安装了 conda ,了解了一下 conda 环境,今天的任务还挺轻松的,但是让我对linux服务器上的软件安装好像认识得更直观了一点点。不过还是不完全明白,我们在服务器上能调用哪些范围内的软件?比如一个组每个同学有自己的账号,但是大家的目录都在课题组目录下,组里别的同学下载安装的软件我也能用,这是为什么呢,我们能调用到哪个层级的目录下的软件是取决于什么呀?

  1. 登录服务器,查看服务器是多少位的,
    bio04@VM-0-6-ubuntu:~$ uname -a
    Linux VM-0-6-ubuntu 4.15.0-118-generic #119-Ubuntu 
    SMP Tue Sep 8 12:30:01 UTC 2020 x86_64 x86_64 x86_64 GNU/Linux
    
  2. 看样子是 64 位的,然后去找miniconda for Linux 64的最新版本,顺利下载到了 biosoft 目录下
    bio04@VM-0-6-ubuntu:~/biosoft$ wget https://mirrors.tuna.tsinghua.edu.cn/anaconda/miniconda/Miniconda3-latest-Linux-x86_64.sh
    --2022-05-11 17:50:14--  https://mirrors.tuna.tsinghua.edu.cn/anaconda/miniconda/Miniconda3-latest-Linux-x86_64.sh
    Resolving mirrors.tuna.tsinghua.edu.cn (mirrors.tuna.tsinghua.edu.cn)... 101.6.15.130, 2402:f000:1:400::2
    Connecting to mirrors.tuna.tsinghua.edu.cn (mirrors.tuna.tsinghua.edu.cn)|101.6.15.130|:443... connected.
    HTTP request sent, awaiting response... 200 OK
    Length: 75660608 (72M) [application/octet-stream]
    Saving to: ‘Miniconda3-latest-Linux-x86_64.sh’
    
    Miniconda3-latest-Linux-x86_64.sh         100%[==================================================================================>]  72.16M  1.19MB/s    in 70s     
    
    2022-05-11 17:51:25 (1.03 MB/s) - ‘Miniconda3-latest-Linux-x86_64.sh’ saved [75660608/75660608]
    
  3. 重要步骤之安装
    输入 bash Miniconda3-latest-Linux-x86_64.sh 之后enter了超多遍过了很多条款,终于到了 Do you accept the license terms? [yes|no]yes 了两下,成功安装!
    ==> For changes to take effect, close and re-open your current shell. <==
    
    If you'd prefer that conda's base environment not be activated on startup, 
    set the auto_activate_base parameter to false: 
    
    conda config --set auto_activate_base false
    
    Thank you for installing Miniconda3!
    ```
    
  4. 重要步骤之激活
    听话地关闭了 Xshell 重新打开,结果发现没注意把conda3 安装到 biosoft 下,一不小心直接安装到了自己的主目录下。问题不大,继续激活。直接在主目录下输入 source ~/.bashrc ,之后在随便哪个文件夹下输入conda都能出来conda的基本信息,不太懂它的作用范围,查了一下安装路径,是在conda里面,所以是在我的主目录下都可以调用的意思吗?
    (base) bio04@VM-0-6-ubuntu:~/tmp$ whereis conda
    conda: /home/bio04/miniconda3/bin/conda /home/bio04/miniconda3/condabin/conda
    
  5. 添加镜像:输入后没有什么反馈,应该就没事了吧
  6. 正式使用conda:
    试了不加 -y 直接 conda install fastqc ,结果就是自己输了一个 y,别的似乎没啥,试了一些 fastqc -help 能看到帮助文档,到这里就安装好 fastqc 啦,也顺便看了看它安装到了哪里
    (base) bio04@VM-0-6-ubuntu:~$ whereis fastqc
    fastqc: /home/bio04/miniconda3/bin/fastqc
    
  7. conda环境
    (base) bio04@VM-0-6-ubuntu:~$ conda info --envs
    # conda environments:
    #
    base                  *  /home/bio04/miniconda3
    
    (base) bio04@VM-0-6-ubuntu:~$ conda create -n rna-seq python=3 fastqc trimmomatic -y
    (base) bio04@VM-0-6-ubuntu:~$ conda info --envs
    # conda environments:
    #
    base                  *  /home/bio04/miniconda3
    rna-seq                  /home/bio04/miniconda3/envs/rna-seq
    
    (base) bio04@VM-0-6-ubuntu:~$ conda activate rna-seq
    (rna-seq) bio04@VM-0-6-ubuntu:~$ conda info --envs
    # conda environments:
    #
    base                     /home/bio04/miniconda3
    rna-seq               *  /home/bio04/miniconda3/envs/rna-seq
    
  8. 见识了一次掉线)
    Socket error Event: 32 Error: 10053.
    Connection closing...Socket close.
    
    Connection closed by foreign host.
    
    Disconnected from remote host(linux) at 19:58:12.
    
    Type `help' to learn how to use Xshell prompt.
    [D:\~]$ 
    

那就到这里吧~

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