基因的FPKM等于各转录本FPKM之和

2019-08-09  本文已影响0人  F_U_N

基因和转录本定量hppRNA STAR-RSEM-Ebseq 流程的四个样本

gene FPKM:

gene_id KO1 KO2 Con1 Con2
DAG1 139.31 112.86 102.46 120.14

Transcription FPKM:

transcript_id gene_id KO1 KO2 Con1 Con2
NM_001177642 DAG1 2.37 2.1 0.95 0.88
NM_001177640 DAG1 1.51 1.62 1.09 0
NM_001177639 DAG1 79.93 70.07 67.33 99.38
NM_001177644 DAG1 0.51 0.21 0.82 0
NM_001177643 DAG1 17.18 13.62 12.34 8.26
NM_001177641 DAG1 0 0 0.13 0.53
NM_001177635 DAG1 2.71 0.22 2.26 0.49
NM_004393 DAG1 32.77 23.12 16.73 10.22
NM_001177634 DAG1 0.62 0.07 0 0.09
NM_001177638 DAG1 0.39 0.19 0 0
NM_001165928 DAG1 0 0.24 0 0
NM_001177637 DAG1 0 1.09 0 0.03
NM_001177636 DAG1 1.32 0.32 0.81 0.26
SUM 139.31 112.87 102.46 120.14

FPKM(gene) = FPKM(transcript 1) + FPKM(transcript 2) + FPKM(transcript 3) + FPKM(transcript n)

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