2022.10.22仍旧艰难的第二篇
首先我真的很爱半途而废所以自己也对坚持写不抱希望！！！嗯嗯！
Akagi, T., I. M. Henry, R. Tao, and L. Comai. ‘A Y-Chromosome-Encoded Small RNA Acts as a Sex Determinant in Persimmons’.Science346, no. 6209 (31 October 2014): 646–50.https://doi.org/10.1126/science.1257225.
花芽怪兽攻击——>
紫色雌花怪兽
黄色雄花怪兽
这篇2014年发表于science的柿子性别决定的文章真是常读常新，初读为作者发现sRNA可以介导同源基因甲基化，从而抑制其表达来调控性别这一神奇的机制而惊叹；再读为其2014年就想到Y-specific k-mer来找Y特异性区段而钦佩；时隔几个月再看，发现其转拟南芥的时候就发现了MeGI dosage-dependent的作用方式，呜呜呜Takashi Akagi等一众老师太神了。
中文：
使用构建的CaMV35S启动子将MeGI独立转入拟南芥中，其中3/11生长迟缓并且雄蕊不育，偶尔产生无功能的花粉状颗粒，通过杂交授粉证明心皮可以产生可育种子。另外8株转基因拟南芥没有生长迟缓并且雄性可育，携带同样过表达载体但显示了MeGI mRNA的不完整剪切，导致了MeGI转录本的低表达。类似的，在烟草中，使用原生启动子转入MeGI基因的5/12的植株显示很低的花粉萌发率和变短的雄蕊，但心皮保持正常。综合起来看，在转基因拟南芥和烟草中观察到的表型和D. lotus 中雌花的表型一致。此外，在柿子和转基因拟南芥中的雄性不育和高MeGI表达之间的联系说明MeGI似乎是依赖于剂量的可导致雄蕊不育的生长抑制子。
英文原文：
Three of 11 Arabidopsis thaliana plants independently transformed with MeGI driven by the constitutive CaMV35s promoter exhibited stunted growth and sterile androecia, occasionally producing nonfunctional pollenlike grains, whereas carpels could produce fertile seeds upon cross-pollination. The other eight A. thaliana transformants carrying the same overpression construct were not stunted and were typically male-fertile but also displayed MeGI mRNA incomplete splicing, resulting in low experssion of the functional MeGI transcripts. Similarly, in N. tabacum, 5 out of 12 plants transformed with MeGI driven by its native promoter exhibited low pollen germination and shorter androecia, whereas capels remained normal. Taken together, the phenotypes observed in transgenic A. thaliana and N. tabacum were consistent with the morphology of female flowers in D. lotus. Furthermore, the association of male sterilation with higher expression of MeGI in persimmon and A. thaliana suggested that MeGI may be s dosage-dependent growth inhibitor that can sterilize androecia.
这一段主要是描述在拟南芥和烟草中过表达MeGI的结果，结合柿子中的自然表型推断MeGI的dosage-dependent的抑制雄性的功能。这段的逻辑非常流畅，前三句使用了parallel structure，结构干净易读；整段的逻辑是A+B+C推论出D，是很好的描述结果+得出结论的一段。
一些结构工整的词：
transformed with MeGI driven by......exhibited......whereas; A
the other displayed......
transformed with MeGI driven by......exhibited......whereas; B
Taken together......(A & C are consistent ); Furthermore......(the consistent suggest that......)
A are consistent with C; the association of A with C
