利用二代测序数据矫正三代Nanopore测序数据
2022-03-22 本文已影响0人
小明的数据分析笔记本
论文
https://genomebiology.biomedcentral.com/articles/10.1186/s13059-020-02244-4
Ratatosk: hybrid error correction of long reads enables accurate variant calling and assembly
https://github.com/DecodeGenetics/Ratatosk
安装
git clone --recursive https://github.com/DecodeGenetics/Ratatosk.git
cd Ratatosk
mkdir build
cd bulid
cmake -DCMAKE_INSTALL_PREFIX=$(pwd) ..
make
make install
运行命令
~/scratch/apps/mingyan/Biotools/Ratatosk/build/bin/Ratatosk correct -v -c 16 -s ERR1878196_1.fastq.gz ERR1878196_2.fastq.gz -l ERR3284704.fastq.gz -o out_long_reads
