转录组数据分析

salmon 1.4.0 基因丰度计算 (二进制版)

2021-06-09  本文已影响0人  胡童远

conda中安装的是salmon 0.13.1,计算基因丰度老出一个bug,如下,因此打算使用最新版试图规避bug,据悉不是版本原因。2020年11出的最新版salmon1.4.0 含二进制版本。

[jointLog] [error] Processing reads : Error reading from the FASTA/Q stream. Minimum return code for left and right read was (-2). Made for left and right read was (-2). Make sure the file is valid.

Github:https://github.com/COMBINE-lab/salmon
下载地址:https://github.com/COMBINE-lab/salmon/releases

下载,安装,测试

win中下载速度更快

 wget -c https://github.com/COMBINE-lab/salmon/releases/download/v1.4.0/salmon-1.4.0_linux_x86_64.tar.gz
tar -zxvf salmon-1.4.0_linux_x86_64.tar.gz
cd salmon-latest_linux_x86_64/bin
./salmon --version
# salmon 1.4.0
./salmon --help
salmon v1.4.0

Usage:  salmon -h|--help or
        salmon -v|--version or
        salmon -c|--cite or
        salmon [--no-version-check] <COMMAND> [-h | options]

Commands:
     index      : create a salmon index
     quant      : quantify a sample
     alevin     : single cell analysis
     swim       : perform super-secret operation
     quantmerge : merge multiple quantifications into a single file
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