StringTie定量出现COV负值报错
2021-02-14 本文已影响0人
信你个鬼
出现如下错误：
image.png
检查对应样本，出现负值：
image.png
14 StringTie transcript 50329404 50396912 1000 - . gene_id "MSTRG.58621"; transcript_id "MSTRG.58621.4"; ref_gene_name "ENSG00000100490"; cov "-2.600490"; FPKM "0.000000"; TPM "0.000000";
14 StringTie exon 50329404 50330181 1000 - . gene_id "MSTRG.58621"; transcript_id "MSTRG.58621.4"; exon_number "1"; ref_gene_name "ENSG00000100490"; cov "-4.341874";
14 StringTie exon 50334565 50334621 1000 - . gene_id "MSTRG.58621"; transcript_id "MSTRG.58621.4"; exon_number "2"; ref_gene_name "ENSG00000100490"; cov "-7.871197";
14 StringTie exon 50338947 50339029 1000 - . gene_id "MSTRG.58621"; transcript_id "MSTRG.58621.4"; exon_number "3"; ref_gene_name "ENSG00000100490"; cov "-4.112955";
14 StringTie exon 50341032 50341232 1000 - . gene_id "MSTRG.58621"; transcript_id "MSTRG.58621.4"; exon_number "4"; ref_gene_name "ENSG00000100490"; cov "-2.416990";
14 StringTie exon 50342132 50342222 1000 - . gene_id "MSTRG.58621"; transcript_id "MSTRG.58621.4"; exon_number "5"; ref_gene_name "ENSG00000100490"; cov "-1.085523";
14 StringTie exon 50344986 50345058 1000 - . gene_id "MSTRG.58621"; transcript_id "MSTRG.58621.4"; exon_number "6"; ref_gene_name "ENSG00000100490"; cov "-0.774802";
14 StringTie exon 50359028 50359149 1000 - . gene_id "MSTRG.58621"; transcript_id "MSTRG.58621.4"; exon_number "7"; ref_gene_name "ENSG00000100490"; cov "-0.659754";
14 StringTie exon 50395701 50396329 1000 - . gene_id "MSTRG.58621"; transcript_id "MSTRG.58621.4"; exon_number "8"; ref_gene_name "ENSG00000100490"; cov "-0.774150";
14 StringTie exon 50396824 50396912 1000 - . gene_id "MSTRG.58621"; transcript_id "MSTRG.58621.4"; exon_number "9"; ref_gene_name "ENSG00000100490"; cov "-1.620675";
然后查到是一个bug，还被反复修来修去：
image.png
image.png
根据报错信息：stringtie negative 能检索到更多信息。
额，但是还不清楚这个软件计算cov咋计算的。
