解决数据上传NCBI时的“multiple BioSamples cannot have identical attributes”错误!

ERROR:

Your table upload failed because multiple BioSamples cannot have identical attributes. You should have one BioSample for each specimen, and each of your BioSamples must have differentiating information (excluding sample name, title, bioproject accession and description). This check was implemented to encourage submitters to include distinguishing information in their samples. If the distinguishing information is in the sample name, title or description, please recode it into an appropriate attribute, either one of the predefined attributes or a custom attribute you define. If it is necessary to represent true biological replicates as separate BioSamples, you might add an 'aliquot' or 'replicate' attribute, e.g., 'replicate = biological replicate 1', as appropriate. Note that multiple assay types, e.g., RNA-seq and ChIP-seq data may reference the same BioSample if appropriate.

上传16s/RNAseq/chip_seq时遇到这个问题怎么办？

搜了一晚上，发现基本没人说到重点！

• 关键问题：

  表格中的绿色是必填项，一定要保证至少一个因子可以区分各个样本（名字除外）。也就是说，除了样本名之外，剩下的必填项中必须还有一个项目是在每个样本都不同的。

• 解决办法：

  如果大家和我一样，除了样本名其他不是一样就是没信息，那么我推荐大家直接修改*lat_lon，也就是经纬度。由于经纬度是保留到小数点后两位，我们直接一次加0.01（在EXCEL往下拉快速填充即可！），对于位置几乎没影响，也可以区分样本，完美解决。

  还有问题的话可以关注我的w x公 号【木舟笔记】，直接后台私信。