canu-2
2019-07-03 本文已影响7人
tobebettergirl
学习网址：
https://canu.readthedocs.io/en/latest/
执行命令
./canu/Linux-amd64/bin/canu
测试数据下载
#pacbio data
curl -L -o pacbio.fastq http://gembox.cbcb.umd.edu/mhap/raw/ecoli_p6_25x.filtered.fastq
#nanopore data
curl -L -o oxford.fasta http://nanopore.s3.climb.ac.uk/MAP006-PCR-1_2D_pass.fasta
组装命令
# For PacBio:
canu \
-p ecoli -d ecoli-pacbio \
genomeSize=4.8m \
-pacbio-raw pacbio.fastq
# For Nanopore:
canu \
-p ecoli -d ecoli-oxford \
genomeSize=4.8m \
-nanopore-raw oxford.fasta
