canu
2019-07-03 本文已影响8人
tobebettergirl
学习网址：
https://github.com/marbl/canu
canu安装
git clone https://github.com/marbl/canu.git
cd canu/src
make -j 8
文献阅读
- Koren S, Walenz BP, Berlin K, Miller JR, Phillippy AM. Canu: scalable and accurate long-read assembly via adaptive k-mer weighting and repeat separation. Genome Research. (2017).
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Canu is able to generate highly continuous assemblies from both PacBio and Nanopore sequencing, but signal-level polishing is required to maximize the final consensus accuracy.
Canu assembly followed by either single-molecule or short-read polishing is an efficient method for generating high-quality assemblies.
Canu currently splits haplotypes into separate contigs wherever the allelic divergence is greater than the post-correction overlap error rate. This threshold is typically 1.5% for recent PacBio data
