生信入门参考资料

生信这样入门可好-公共版本

2018-07-01  本文已影响237人  因地制宜的生信达人

首先你需要了解一些测序基本知识,组学基本知识,这个主要靠自己看,还有平时多积累。
http://www.biotrainee.com/forum-2-1.html 这个版块我列了一下,然后就是不懂的名词多搜索,多熟记。

然后你需要掌握一门编程语言,可以从http://www.biotrainee.com/forum-90-1.html 里面的题目开始,当然你需要先来一些简单,你先看这个,http://www.biotrainee.com/thread-834-1-1.html 编程学会怎么着也得两三个月了。

如果是linux,不妨看看实验楼的练习:

  1. 全部课程 — >Linux新手入门计算机专业课 — > Linux 基础入门(新版)

最后,你需要实战几个组学项目。看这个,http://www.biotrainee.com/forum-108-1.html 这个取决于你的悟性了。
关于文献相关:
首先看 5 篇综述 并翻译: WES/RNA-seq/CHIP-seq/miRNA-seq/lncRNA-seq
Practical Guidelines for the Comprehensive Analysis of ChIP-seq Data : http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3828144/
A survey of tools for variant analysis of next-generation genome sequencing data : http://bib.oxfordjournals.org/content/15/2/256.short

A survey of best practices for RNA-seq data analysis : http://genomebiology.biomedcentral.com/articles/10.1186/s13059-016-0881-8
Online resources for miRNA analysis : http://www.sciencedirect.com/science/article/pii/S0009912013000994
Long non-coding RNA discovery pipeline : http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0025915

然后看5篇测序数据分析例子:
miRNA-seq :
RNA expression profiling of human iPSC-derived cardiomyocytes in a cardiac hypertrophy model. : http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0108051
CHIP-seq :
CARM1 Methylates Chromatin Remodeling Factor BAF155 to Enhance Tumor Progression and Metastasis http://www.cell.com/cancer-cell/abstract/S1535-6108(13)00536-9
WES :
Clinical Whole-Exome Sequencing for the Diagnosis of Mendelian Disorders http://www.nejm.org/doi/full/10.1056/NEJMoa1306555#t=article
LncRNA:
Integration of Genome-wide Approaches Identifies lncRNAs of Adult Neural Stem Cells and Their Progeny In Vivo http://www.sciencedirect.com/science/article/pii/S1934590913000982
RNA-seq:
RNA-Seq Transcriptome Profiling Identifies CRISPLD2 as a Glucocorticoid Responsive Gene that Modulates Cytokine Function in Airway Smooth Muscle Cells

然后看5篇芯片数据分析例子:
lncRNA : http://pubmedcentralcanada.ca/pmcc/articles/PMC3691033/

有参组学(全基因组,全外显子组学,转录组学,表观)的几个NGS测序数据分析的表现形式的异同点,视频在链接:http://pan.baidu.com/s/1jIQFGSA 密码:48uj

TCGA大文章的打包下载链接:http://pan.baidu.com/s/1bpaLmTT

我做博客3年来录制的所有免费视频: https://pan.baidu.com/s/1pKkA4Q3 密码:8xxd

上一篇下一篇

猜你喜欢

热点阅读