NGS各种组学建库原理(图解)
2020-08-08 本文已影响0人
pythonic生物人
本文分享llumina官网上的各种组学建库原理图,分三个方向:Seq—DNA(DNA层面)、Seq—RNA(RNA层面)、Seq—Single-Cell(单细胞层面)。
本文将了解到什么?
1、DNA层面各组学建库原理图解:Seq—DNA
DNA variants
Low levels of DNA
Epigenetics
DNA–protein interactions
Protein–protein interactions
2、RNA层面各组学建库原理图解:Seq—RNA
RNA transcription
RNA–protein interactions
Low levels of RNA
RNA modifications
RNA structure
3、单细胞层面各组学建库原理图解:Seq—Single-Cell
Low-level RNA detection
Low-level DNA detection
Integrated DNA and RNA analysis
1、DNA层面:Seq—DNA
具体分如下几个方向
- DNA variants
- Low levels of DNA
- Epigenetics
CAB-Seq
![](https://img.haomeiwen.com/i15446909/5d50c72f24bf9fe3.png)
![](https://img.haomeiwen.com/i15446909/f07d387d1437de77.png)
- DNA–protein interactions
ChIP-Seq
![](https://img.haomeiwen.com/i15446909/1b169700e10e5440.png)
ATAC-Seq
![](https://img.haomeiwen.com/i15446909/2abb5cf40a815d3d.png)
![](https://img.haomeiwen.com/i15446909/6c475c42eae6e70f.png)
- Protein–protein interactions
![](https://img.haomeiwen.com/i15446909/cce7332b8b1f57db.png)
2、RNA层面: Seq—RNA
分以下几类:
- RNA transcription
- RNA–protein interactions
- Low levels of RNA
UMI Method
- RNA modifications
- RNA structure
3、单细胞层面:Seq—Single-Cell
可分以下几个方面:
- Low-level RNA detection
- Low-level DNA detection
- Integrated DNA and RNA analysis
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