因为权限问题安装失败

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大意是：错误 1303。安装程序没有足够的权限来访问C:\programData\Microsoft\Windows\Start Menu\Programs\Autodesk这个目录。安装无法继续。以管理员身份登录，或与您的系统管理员联系。

不会吧！我Windows 8还有这嗜好？！立马启动内置的 administrator 后，重启电脑，使用 administrator 大法安装。居然还是这脸色！神马玩意？！

仔细研究，多次重启，终于搞定！办法如下：

1、打开C:\programData\Microsoft\Windows\Start Menu\文件夹，找到Programs；
2、右击Programs-->属性，切换到“安全”标签；
3、点击“编辑”按钮-->“添加”-->“高级”-->“立即查找”，在搜索结果栏中找到SYSTEM-->“确定”-->“确定”；
4、然后在次安装就可以了

识别不了USB

在运行中输入services.msc
找到VMwaer USB 开头的文件，全部点启动

安装VMware tools

打开虚拟机后，点击 “设置——安装VMware tools”，然后点 “是”，然后虚拟机里面就有了VMware tools 的压缩包，移到home目录下

tar -zxvf VMwareTools-9.2.2-893683.tar.gz
cd vmware-tools-distrib
sudo perl vmware-install.pl

就可以了

安装aspera

推荐：Aspera工具安装与使用

wget -c https://d3gcli72yxqn2z.cloudfront.net/connect/bin/ibm-aspera-connect-3.8.1.161274-linux-g2.12-64.tar.gz
tar -zxvf ibm-aspera-connect-3.8.1.161274-linux-g2.12-64.tar.gz
#2018.7.19
mkdir biosoft
mv ibm-aspera-connect-3.8.0.158533-linux-g2.12-64.sh biosoft/
cd biosoft/
bash ibm-aspera-connect-3.8.0.158533-linux-g2.12-64.sh
cd
echo export PATH="/home/u1013/.aspera/connect/bin:$PATH" >>.bashrc

使用aspera下载文件

for i in `seq 00 03`
do
        ascp -T -l 200M -i ~/.aspera/connect/etc/asperaweb_id_dsa.openssh --host=ftp-private.ncbi.nlm.nih.gov --user=anonftp --mode=recv /sra/sra-instant/reads/ByRun/sra/SRR/SRR402/SRR40223$i/SRR40223$i.sra ./
done

在使用的过程中可能遇到网络问题
参考：http://download.asperasoft.com/download/docs/proxy/1.2.2/admin_linux/webhelp/dita/troubleshooting.html
Session Stop (Error: Client unable to connect to server (check UDP port and firewall))

If you get this error, check the following:

1. Ensure that IP forwarding is enabled. IP forwarding must be enabled and is enabled automatically when FASP Proxy is installed. To confirm, run the following command:

        cat /proc/sys/net/ipv4/ip_forward 

    If the command returns 1, IP forwarding is enabled. If it returns 0, it is not. IP forwarding can be enabled manually by setting the **net.ipv4.ip_forward** line in **/etc/sysctl.conf** as follows:

# Controls IP packet forwarding
      net.ipv4.ip_forward=1

To activate changes to /etc/sysctl.conf, run the following:

  /sbin/sysctl -p /etc/sysctl.conf

2. If the error still occurs when IP forwarding is on, open your aspera.conf file and turn off source-port filtering as follows. By default, source-port filtering is enabled.

...
<rproxy>
  <enabled>false</enabled> 
  ... 
  <rules>
    <rule>
      ... 
      <src_port_filtering>false</src_port_filtering> 
    </rule>
  </rules>
</rproxy>
...

If the same timeout errors still occur when source-port filtering is disabled, this generally indicates that traffic is being blocked at a firewall.

For more information about source-port filtering, see Source-Port Filtering.

安装conda

wget -c https://repo.continuum.io/miniconda/Miniconda3-latest-Linux-x86_64.sh
sh Miniconda3-latest-Linux-x86_64.sh #全部选择yes，就将conda添加到环境变量了，有时需要使用bash代替sh
source ~/.bashrc

添加channels

conda config --add channels r
conda config --add channels defaults
conda config --add channels conda-forge
conda config --add channels bioconda

添加国内channels

conda config --add channels https://mirrors.tuna.tsinghua.edu.cn/anaconda/cloud/conda-forge/
conda config --add channels https://mirrors.tuna.tsinghua.edu.cn/anaconda/pkgs/free/
conda config --add channels https://mirrors.tuna.tsinghua.edu.cn/anaconda/cloud/bioconda/

上下左右键变成ABCD

cp /etc/vim/vimrc ~/.vimrc

将软件添加到环境变量

export PATH=$PATH:/opt/au1200_rm/build_tools/bin #就是将软件的bin目录添加到环境变量

或是直接在bashrc里面进行修改

vi .bashrc
export PATH="/opt/au1200_rm/build_tools/bin:$PATH"

然后source一下

source .bashrc