10xgenomics做scRNA-seq
2019-01-02 本文已影响30人
njmujjc
PATH=/media/pc/disk2/jjc/2018-9-5-scRNA-seq/cellranger-2.2.0:$PATH
##fastq文件名字要改为SRR6230316_S1_L001_R1_001.fastq.gz
cellranger count --id=xx --transcriptome=/media/pc/disk2/jjc/2018-9-5-scRNA-seq/refdata-cellranger-mm10-2.1.0 --fastqs=/media/pc/disk2/jjc/2018-9-5-scRNA-seq/2018-9-5-scRNA-seq/NP_2_fastq --sample=SRR6230316 --localcores=20
cellranger aggr --id=JJC --csv=JJC_libraries.csv--normalize=none
