这篇发表在Aging-US的杂志，比较常规，但还是有一定借鉴意义的。

PMID: 34115610
发表杂志：Aging-US
PMID:34115610

启发点

• 收集5对LUAD标本，提取RNA进行验证。
• 一致性聚类后，CIBERSORT，ESTIMATE和GSVA分析。

知识点

FRGs:Ferroptosis-Related Genes 铁死亡相关基因，作者根据以往文献收集整理60个FRGs基因。

整体思路

整体思路

结果

1.FRGs差异分析

Fig.2

2.一致性聚类

一致性聚类，聚类结果和预后相关，且与免疫评分及CIBERSORT免疫细胞浸润有关，GSVA结果同样证实FRGs基因很重要。

Fig.3
FigureS1

看看作者如何解释GSVA结果

Heat maps showed that upregulation of the malignant hallmarks of tumors, including the Wnt/β-catenin signaling pathway, p53 pathway, KRAS signaling pathway, TGF-β signaling pathway, and PI3K/AKT/mTOR signaling pathway, was dynamically related to cluster 1 (Supplementary Figure 1). According to the above results, we can infer that these signaling pathways may be involved in the different TMEs of clusters 1 and 2. These results provide further support that variations in FRG expression affect the prognosis of LUAD patients.

3.模型构建及验证

Fig.4

4.模型周边

Fig.5

5.模型基因周边

5.1 TIMER分析Gene alterations与免疫细胞浸润

Fig.6

5.2 临床标本验证

Fig.7
参考链接：
A novel ferroptosis-related gene signature for prognostic prediction of patients with lung adenocarcinoma