测序质控软件-trim_galore安装(2018-05-25)
1. 源代码下载:https://github.com/FelixKrueger/TrimGalore
2. 查看安装日志:
Installation
Trim Galore!is a a Perl wrapper around two tools: Cutadapt and FastQC. To use, ensure that these two pieces of software are available and copy the trim_galore script to a location available on the PATH.
For example:
#Check that cutadapt is installed
cutadapt --version
#Check that FastQC is installed
fastqc -v
#Install Trim Galore!
curl -fsSL https://github.com/FelixKrueger/TrimGalore/archive/0.4.5.tar.gz -o trim_galore.tar.gz tar xvzf trim_galore.tar.gz
mv TrimGalore-0.4.5/trim_galore /usr/bin
大概是说安装这个软件上之前需要安装Fastqc和cutadapt两个软件,Fastqc 使用conda 就可以安装。
3. cutadapt下载源代码:https://github.com/marcelm/cutadapt
安装:
git clone https://github.com/marcelm/cutadapt/
cd cutadapt
python setup.py build_ext -i
需要下载cython,下载安装还是无法进行
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没跑通,后面再看吧
4.使用conda安装cutadapt
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5.安装trim_galore
export PATH=path$PATH
已安装